|
KMID : 0380020190340030192
|
|
Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 3 p.192 ~ p.198
|
|
|
Biological Activity of Supercritical Fluid Extracts from the Braches of Alnus japonica
|
|
Ha Eun-Ju
Choi Sun-Eun
|
|
|
Abstract
|
|
|
|
The branches of Alnus japonica have been used in oriental traditional medicine practice for the formulation of medicines as the remedies for fever, hemorrhage, diarrhea, gastroenteric disorder, lymphatic disease, and cancers. In this study, the anti-bacterial, anti-oxidant, anti-inflammatory, and anti-allergic activities of a supercritical extract of branches of Alnus japonica (AJSCF) were investigated by in vitro assays. To investigate the antioxidant and anti-inflammatory effects of a supercritical extract of branches of AJSCF, ABTS radical scavenging activities and inhibitory activity against nitric oxide production in lipopolysaccharide (LPS) stimulated RAW 264.7 cells were examined. Furthermore, anti-bacterial activities were examined. AJSCF on immunoglobulin E (IgE)-induced allergic inflammation in RBL-2H3 cells. In conclusion, it was observed that dose-dependent increase in AJSCF concentration led to an increase in the ABTS radical scavenging activity. The AJSCF showed low cytotoxicity at 50ug/mL concentration, which was the highest concentration of the examination. The cells stimulated with LPS produced more NO than normal control cells; however, cells treated with supercritical fluid extracts of branches of Alnus japonica inhibited NO production in a concentration-dependent manner. In addition, AJSCF showed the highest antifungal activity and concentration-dependent anti-allergic activity. The results suggest that AJSCF might be developed as a potent anti-fungal, anti-oxidant, anti-inflammatory, and anti-allergic agent for the production of novel and functional cosmetic materials.
|
|
|
KEYWORD
|
|
|
Alnus japonica, supercritical fluid, anti-fungal, antioxidative, anti-inflammatory, anti-allergic
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|